Your Name _______________________                    First Unknown

Unknown Number _______             Species: ____________________     ____________________

Broth Characteristics          ____________________________________________________________

Circle your results:

Test	Positive		Negative
Motility	Spread away from stab mark		Near stab mark.
Glucose Broth – Acid	Yellow		Orange or Red
TSI – Only uses glucose	Red Slant /Yellow Butt		Other than  Red/Yellow
Glucose Broth – Gas	Bubble in Durham Tube		No Bubble
TSI – Gas	Split Agar, Bubbles in Agar		No Split or Bubbles
TSI – Lactose/Sucrose Acid	Yellow Slant/Yellow Butt		Other than Yellow/Yellow
Lactose Broth - Acid	Yellow		Red or Orange
Lactose Broth - Gas	Bubble in Durham Tube		No Bubble
MacConkey Gram Positive	Absence of Colonies		Presence of Colonies **
MacConkey Lactose	Red, Pink Colonies		Colonies not Red, Pink
Gelatin Hydrolysis	Liquid after ice, Room Temp.		Solid after ice, Room Temp.
TSI - Hydrogen Sulfide	Black		Not Black
Catalase – Hydrogen Peroxide	Bubbles		No Bubbles
Citrate Utilization	Any Blue (colony growth)		Green, no colony growth
Oxidase Deep	Pink/Purple at 10 to 30 seconds		Not Pink/Purple
Indole Deep	Red at 10 to 30 seconds		Not Red
Thioglycolate Tube	Aerobic	Anaerobic	Facultatively Anaerobic

**Don’t circle if colonies are not present.

Draw Pictures

Streak Plate (nutrient agar or brain/heart agar)

Colony Shape ___________________           Colony Margin ______________ 

Colony Elevation _________________    

 Other Colony Characteristics ______________________

Gram Stain at 100x with oil

Color ______________          Cell Shape ____________      Cell Arrangement ___________

Gram Positive or Gram Negative? ____________